Allelotype analysis of esophageal squamous cell carcinoma.

作者: Takashi Wagata , Ichio Shibagaki , Masayuki Imamura , Mituo Ikenaga , Yutaka Shimada

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摘要: In previous studies, we have shown that allelic loss on chromosome 17p, which the p53 gene is located, very frequent, and loss-of-function mutations of are closely associated with tumorigenesis esophageal cancer. this study, performed allelotype analysis to investigate whether other tumor suppressor genes also involved in Using 55 polymorphic DNA markers covering every autosomal arm except 13p, 21p, 22p, restriction fragment length polymorphism was 36 squamous cell carcinomas (ESCs) their adjacent normal tissue samples. Frequent heterozygosity (LOH) > 30% informative cases observed chromosomes 3p (41.1%), 5q (52.6%), 6p (30.4%), 8p (33.3%), 9p (35.7%), 9q (30.8%), 11p (32.4%), 13q (52.7%), 17p (55.2%), 17q 18q (45.7%), 19q (30.4%). Among these, LOH 5q, 13q, previously reported ESC considered involve APC, RB, p53, DCC genes, respectively. However, our deletion revealed region commonly lost did not include locus, suggesting a possible than ESC. We screened 60 primary tumors 20 cultured lines for mutation APC within cluster exon 15, where "hot spot" somatic colorectal pancreatic cancers thought be. could find any despite high frequency 5q. analyzed relationship between clinicopathological data found poor prognosis.

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