Assessment of specific mRNA levels in cartilage regions in a lapine model of osteoarthritis.

摘要: Osteoarthritis (OA) is the most common form of arthritis and patients with meniscal ligament injuries knee are at high risk to develop disease. The purpose this study was evaluate molecular structural changes occurring in four articular cartilage (AC) regions from knees anterior cruciate (ACL)-transected rabbits 3 8 weeks post-surgery. Rabbit AC lateral medial femoral condyles (LFC MFC) as well tibial plateau (MTP LTP) were processed for histology semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis a subset relevant molecules (collagen II, aggrecan, biglycan, decorin, fibromodulin, MMP-1,-3,-13, TIMP-1). While severe histological observed MTP starting early post-ACL transection based on Mankin scores, examination demonstrated progression osteoarthritic MFC In contrast, very few within both LFC LTP, these did not worsen increasing time after surgery. water content increased significantly MTP. Significant decreases DNA MFC, LTP transection. Total RNA yields elevated transection, while compartment total unchanged following ACL Analysis mRNA levels matrix molecules, proteinases proteinase inhibitors, by RT-PCR significant region-specific level These results show that complex molecular, occur time-dependent.