Identification of Gangliosides GD1b and GT1b as Receptors for BK Virus

摘要: Gangliosides have been shown to be plasma membrane receptors for both murine polyomavirus and SV40, while JC virus uses serotonin receptors. In contrast, little is known of the receptor entry pathway BK (BKV), which can cause severe disease in immunosuppressed bone marrow renal transplant patients. Using sucrose flotation assays, we investigated BKV binding interaction with human erythrocyte membranes determined that this was dependent on a neuraminidase-sensitive, proteinase K-resistant molecule. found interact gangliosides GT1b GD1b. The terminal α2-8-linked disialic acid motif, present these gangliosides, likely important interaction. We also addition GD1b LNCaP cells, are normally resistant infection, made them susceptible virus. addition, interacted extracted from endoplasmic reticulum (ER) infection blocked by brefeldin A, interferes transport ER Golgi apparatus. These data demonstrate as passes through way nucleus.