Fluorigenic method for the assay of proteinase activity with the use of 4-methylumbelliferyl-casein.

作者: H Khalfan , R Abuknesha , D Robinson

DOI: 10.1042/BJ2090265

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摘要: A method for the preparation of casein labelled with 4-methylumbelliferyl fluorophore is described, and product was used as a fluorigenic macromolecular substrate sensitive assay activity proteinases. Nanogram quantities trypsin, chymotrypsin, elastase cathepsin D can be detected, but unaffected by B.

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