Human Postmortem Tissue: What Quality Markers Matter?

摘要: Postmortem human brain tissue is used for the study of many different diseases. A key factor in conducting postmortem research quality tissue. Unlike animal tissue, whose condition at death can be controlled and influenced, only collected naturalistically. This introduces potential confounds, based both on pre- conditions, that may influence its ability to yield accurate results. The traditionally recognized confounds reduce are agonal factors (e.g., coma, hypoxia, hyperpyrexia time death), long interval (PMI). We measured parameters over 100 cases from sources correlated them with RNA (as indicated by Integrity Number (RIN)) protein level representative proteins). Our results show most sensible indicator RIN there a good correlation between pH. No developed levels aforementioned factors. Moreover, even when was degraded, remained stable. However, these correlations did not prove true under all circumstances thawed surgical tissue), yielded unexpected indicators. These data also suggest source Medical Examiner's office represent high quality.