Altered vimentin protein expression in human dermal microvascular endothelial cells after ultraviolet or intense pulsed light treatment

摘要: Background Endothelial cell senescence is closely related to tissue aging and age-related vascular disease. Detailed pathophysiology essential biomarkers of skin are not well known. A recent report suggests that advanced glycosylation end products, especially Nϵ-(carboxymethyl)lysine (CML) modification vimentin, accelerate the process. Objectives To identify protein in skin. Materials Methods Proteomics analysis was performed differentially expressed proteins human dermal microvascular endothelial cells (HDMEC) treated with ultraviolet (UV) or intense pulsed light (IPL). Proteome maps UV-treated, IPL-treated, untreated HDMEC were constructed, identification altered spots by matrix-assisted laser desorption/ionization mass spectrometry. Differential expression glycation vimentin found this approach further examined fluorescence-activated sorting. Results Twenty-two identified. Among them, specifically up-regulated UV-treated HDMECs. On other hand, it down-regulated after IPL. Increased CML-vimentin during culture (Passage 6 vs. 12) noted, effect reversed IPL treatment. Conclusion Vimentin CML should be useful markers for senescence, as evaluating level aging. Also, targeting increased its products could present a target treatment Lasers Surg. Med. 46:431–438, 2014. © 2014 Wiley Periodicals, Inc.