Metabolic engineering of a pentose metabolism pathway in ethanologenic Zymomonas mobilis

作者: M. Zhang , C. Eddy , K. Deanda , M. Finkelstein , S. Picataggio

DOI: 10.1126/SCIENCE.267.5195.240

关键词:

摘要: The ethanol-producing bacterium Zymomonas mobilis was metabolically engineered to broaden its range of fermentable substrates include the pentose sugar xylose. Two operons encoding xylose assimilation and phosphate pathway enzymes were constructed transformed into Z. in order generate a strain that grew on efficiently fermented it ethanol. Thus, anaerobic fermentation ethanol achieved through combination Entner-Doudoroff pathways. Furthermore, this both glucose xylose, which is essential for economical conversion lignocellulosic biomass

参考文章(20)
L O Ingram, T Conway, D P Clark, G W Sewell, J F Preston, Genetic engineering of ethanol production in Escherichia coli. Applied and Environmental Microbiology. ,vol. 53, pp. 2420- 2425 ,(1987) , 10.1128/AEM.53.10.2420-2425.1987
M E Burnett, J Liu, T Conway, Molecular characterization of the Zymomonas mobilis enolase (eno) gene Journal of Bacteriology. ,vol. 174, pp. 6548- 6553 ,(1992) , 10.1128/JB.174.20.6548-6553.1992
Anthony A. DiMarco, Antonio H. Romano, d-Glucose Transport System of Zymomonas mobilis Applied and Environmental Microbiology. ,vol. 49, pp. 151- 157 ,(1985) , 10.1128/AEM.49.1.151-157.1985
Mia Callens, Hilda Kersters-Hilderson, Omer Van Opstal, Clement K. De Bruyne, Catalytic properties of d-xylose isomerase from Streptomyces violaceoruber Enzyme and Microbial Technology. ,vol. 8, pp. 696- 700 ,(1986) , 10.1016/0141-0229(86)90069-4
Hugh G. Lawford, A new approach to improving the performance ofZymomonas in continuous ethanol fermentations Applied Biochemistry and Biotechnology. ,vol. 17, pp. 203- 219 ,(1988) , 10.1007/BF02779158
Peter K�tter, Ren� Amore, Cornelis P. Hollenberg, Michael Ciriacy, Isolation and characterization of the Pichia stipitis xylitol dehydrogenase gene, XYL2, and construction of a xylose-utilizing Saccharomyces cerevisiae transformant. Current Genetics. ,vol. 18, pp. 493- 500 ,(1990) , 10.1007/BF00327019
Manee Tantirungkij, Noriyuki Nakashima, Tatsuji Seki, Toshiomi Yoshida, Construction of xylose-assimilating Saccharomyces cerevisiae Journal of Fermentation and Bioengineering. ,vol. 75, pp. 83- 88 ,(1993) , 10.1016/0922-338X(93)90214-S
J. Bailey, Toward a science of metabolic engineering Science. ,vol. 252, pp. 1668- 1675 ,(1991) , 10.1126/SCIENCE.2047876