Use of nuclear magnetic resonance and gas-liquid chromatography for the study of microbial nitrile-hydratases and amidases
作者： K. Bui , H. Fradet , A. Thiéry , M. Maestracci , A. Arnaud
DOI: 10.1016/S0003-2670(00)81525-1
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摘要: Abstract Some microbial strains can hydrolyse many nitriles to the corresponding organic acids by means of two enzymes: a nitrile-hydratase which hydrates nitrile amide, and an amidase hydrolyses amide acid. Two methods are proposed for assay enzymatic activities: proton magnetic resonance spectrometry (n.m.r.) gas-liquid chromatography (g.l.c.). For these activities (nitrile-hydratase amidase), g.l.c, was better, because it more precise much sensitive than n.m.r, method. The examples acetonitrile acetamide described in detail.
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