Characterization of underivatized lipid biomarkers from microorganisms with pyrolysis short-column gas chromatography/ion trap mass spectrometry.

摘要: A microvolume Curie-point pyrolysis short-column (5 m) gas chromatography/mass spectrometry (Py-Gc/MS) procedure was developed for the characterization of various lipid moieties in microorganisms. High linear flow rates (approximately 175 cm/s) characterized GC conditions order to effect an efficient chromatographic transfer and elution underivatized diglycerides monoglycerides, small modifications were necessary ion trap MS system it accommodate relatively high load. During a typical analysis run anhydrodiacylglycerides eluted within 5-6-min time frame. Gram-positive bacilli Gram-negative species differentiated from each other by patterns their components. In spite complexity analyte, straightforward visual achieved with aid simple computerized data display procedures. These procedures included examination (1) total current (TIC) profiles region reconstructed chromatogram, (2) integrated mass spectrum this region, (3) selected chromatograms (RICs), (4) RIC intensity distributions, (5) corresponding spectra. An appealing aspect reduction is that most can be accomplished visually without requiring pattern recognition techniques.