Analysis of 10B antitumoral compounds by means of flow-injection into ESI-MS/MS

摘要: Boron neutron capture therapy (BNCT) is a promising binary treatment for cancer. BNCT based on the ability of nonradioactive isotope 1 0 B to capture, with very high probability, thermal neutrons. This nuclear reaction results in two particles (an alpha and lithium nucleus). The have biological effectiveness, which limited tissue approximately diameter one cell. If can be tumor cell, physical characteristic opens up possibility selectively destroy cancer cells, while sparing surrounding healthy tissue. Quality control B-containing compounds their distribution at present are important, different analytical methods been developed, such as time-of-flight secondary ion mass spectrometry (TOF-SIMS), electron energy loss (EELS), prompt gamma analysis inductively coupled plasma-optical emission (ICPOES). These allow analyses B, but it not possible characterize specific molecular containing B. For this reason, we propose fast quantitative method that permits determination closo-undecahydro-1-mercaptododecaborate (BSH) boron-phenylalanine (BPA) eventual metabolites. In particular, detected by means flow-injection electrospray tandem (FI/ESI-MS/MS). approach allows identification compounds, BSH BPA, using spectrometry, also (c.v. ′4.7%; n = 5; linear range 10-10000 ng/ml). Furthermore, were actual sample (urine plasma, diluted 10 000- 1000-fold, respectively) injecting small volume (1 μl) samples.