Proof-of-concept: 3D bioprinting of pigmented human skin constructs

摘要: Three-dimensional (3D) pigmented human skin constructs have been fabricated using a 3D bioprinting approach. The are obtained from three different types of cells (keratinocytes, melanocytes and fibroblasts donors) they exhibit similar constitutive pigmentation (pale pigmentation) as the donors. A two-step drop-on-demand strategy facilitates deposition cell droplets to emulate epidermal melanin units (pre-defined patterning keratinocytes at desired positions) manipulation microenvironment fabricate biomimetic hierarchical porous structures found in native tissue. bioprinted compared by conventional manual-casting approach; in-depth characterization both has indicated that higher degree resemblance tissue term presence well-developed stratified layers continuous layer basement membrane proteins manually-cast samples. approach development vitro for potential toxicology testing fundamental biology research.