Scale-up of counter-current chromatography: demonstration of predictable isocratic and quasi-continuous operating modes from the test tube to pilot/process scale.

摘要: Predictable scale-up from test tube derived distribution ratios and analytical-scale sample loading optimisation is demonstrated using a model system of benzyl alcohol p-cresol in heptane:ethyl acetate:methanol:water phase with the new 18 L Maxi counter-current chromatography centrifuge. The versatility having liquid stationary its high capacity flexible operating modes at two different scales by separating concentrating target compounds mixture caffeine, vanillin, naringenin carvone quasi-continuous technique called intermittent extraction.