A porcine deep dermal partial thickness burn model with hypertrophic scarring

摘要: We developed a reproducible model of deep dermal partial thickness burn injury in juvenile Large White pigs. The contact is created using water at 92 °C for 15 s bottle with the bottom replaced plastic wrap. depth was determined by histopathologist who examined tissue sections 2 and 6 days after blinded manner. Upon creation, circular wound area white eschar hyperaemic zone around border. Animals were kept weeks or 99 to examine healing process. wounds took between 3 5 complete re-epithelialisation. Most contracted, purple, hypertrophic scars. On measurement, burned skin approximately 1.8 times that control week 2.2 thicker than injury. have various methods assess wounds, including digital photographic analysis, organising granulation tissue, immunohistochemistry, electron microscopy tensiometry. Immunohistochemistry showed our porcine scar appears similar human scarring. development this allows us test compare different treatments on wounds.