Donkey’s milk protein fractions characterization

摘要: Abstract Donkey’s milk may be considered a valid alternative, for infant nutrition, to powdered milks, soybean or other formulas, since its composition in lipids and proteins is very close human milk. The aim of the present study characterize “donkey’s milk” product, especially with regard protein fractions. Three different chromatographic approaches donkey’s separation are described, based on their isoelectric points, hydrophobicity molecular mass. obtained were further separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis identified N-terminal sequencing. α S1 - β-caseins, lysozyme, α-lactalbumin, β-lactoglobulin identified. Furthermore, content β-lactoglobulin, lysozyme was determined reversed-phase HPLC method. α-lactalbumin 3.75 1.8 mg/ml, respectively (mean values). showed nutritional properties characterized low casein high (1.0 mg/ml) compared kinds milks.