Possible incorporation of phosphoserine into globin readthrough protein via bovine opal suppressor phosphoseryl-tRNA
作者： Takaharu Mizutani , Yoshio Tachibana
DOI: 10.1016/0014-5793(86)80032-1
关键词:
摘要: Suppressor [32P]phosphoseryl-tRNA, prepared using bovine seryl-tRNA synthetase and ATP:seryl-tRNA phosphotransferase, was mixed with rabbit reticulocyte lysates containing endogenous hemoglobin mRNA having the termination codon UGA (opal). The Chromatographic pattern of lysate on Sephacryl S-200 showed that radioactivity [32P]Pjphosphate in hot trichloroacetic acid-precipitate (phosphoprotein) eluted at position between mature globin subunits. phosphoprotein, obtained by chromatography S-200, moved to corresponding readthrough protein SDS-PAGE. analyses hydrolyzate phosphoprotein presence phosphoserine protein. These results suggest animal opal suppressor tRNA functions vitro transfer (opal) mRNA.
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