Transfection of wtp53 and Rb94 Genes Into Retinoblastomas of Nude Mice by Ultrasound-Targeted Microbubble Destruction

摘要: Using ultrasound-targeted microbubble destruction (UTMD), we transfected both wild-type p53 (wtp53) and Rb94 genes into retinoblastomas (RBs) of nude mice to investigate the inhibitory role these two in RB development. The 40 tumor-bearing mice, which had been established by sub-retinal injection an HXO-Rb44 cell suspension, were randomly divided five groups: blank control group (C); plasmid (M); wtp53 (p53); (Rb94); + (p53 Rb94). For preparation DNA-loaded microbubbles, a pre-determined amount plasmid, pVIVO1-p53, pVIVO1-Rb94 or pVIVO1-p53-Rb94 was added mixed with microbubbles. Then, microbubbles respectively animal model UTMD. Vascular endothelial growth factor level microvessel density tumor determined immunohistochemical staining. Apoptosis tissues detected terminal deoxynucleotidyl transferase dUTP nick end labeling Expression at gene protein levels RT-PCR (reverse transcription polymerase chain reaction) Western blot, respectively. Transfection greater effects on development resulted lower vascular factor, more obvious apoptosis than single-gene transfection (p < 0.05). results indicate that UTMD strongly inhibited single gene.