A monoclonal antibody which recognizes a glycosaminoglycan epitope in both dermatan sulfate and chondroitin sulfate proteoglycans of human skin

摘要: Studies have been initiated to identify various cell surface and matrix components of normal human skin through the production characterization murine monoclonal antibodies. One such antibody, termed PG-4, identifies both antigens in extracts foetal adult as dermatan sulfate proteoglycans, decorin biglycan, chondroitin proteoglycan versican. Treatment proteoglycans with chondroitinases completely abolishes immunoreactivity for all these which suggests that epitope resides within their glycosaminoglycan chains. Further evidence carbohydrate nature derives from competition studies where protein-free chains shark cartilage react strongly; however, bovine tracheal fail exhibit a significant reactivity, an indication epitope, although present some chains, does not consist random 4- or 6-sulfate disaccharides. The presence on was also demonstrated using assays. Thus, PG-4 belongs class antibodies recognize native epitopes located It differs previously described this it proteoglycans. These characteristics make useful antibody probe total population skin.