The influence of Ni(II) on surface antigen expression in murine macrophages.

摘要: Abstract Biomedical alloys may release nickel ions during corrosion phenomena and, in addition to their interaction with oral tissues, these also influence characteristic properties of the immune system cells. The aim this study was evaluate effect chloride on expression functionally distinct surface antigens murine RAW macrophages. CD14, CD40, MHC class I, II, CD80, CD86, CD54 analyzed by flow cytometry. bacterial endotoxin lipopolysaccharide (LPS) used as a positive control induce antigen expression. Cells were stimulated NiCl 2 (0.1 and 0.5 m m ) presence absence LPS or 25 μg/ml). After exposure periods 6, 24 48 h, caused time- dose-dependent increase all antigens. CD14 up-regulated 0.1 μg/ml about 10-fold after 24 h 100-fold 48 h. alone between 2- and 4-fold, while cells LPS, 0.1 m effective only CD40 I. Moreover, even inhibited LPS-induced antigens, except for CD54, which still significantly up-regulated. These results show that is able an up-regulation expression, but high concentration impair essential functions macrophages LPS.