Phospholipid–Sepiolite Biomimetic Interfaces for the Immobilization of Enzymes
作者： Bernd Wicklein , Margarita Darder , Pilar Aranda , Eduardo Ruiz-Hitzky
DOI: 10.1021/AM201000K
关键词:
摘要: Biomimetic interfaces based on phosphatidylcholine (PC) assembled to the natural silicate sepiolite were prepared for stable immobilization of urease and cholesterol oxidase enzymes. This is an important issue in practical advanced applications such as biocatalysis or biosensing. The supported lipid bilayer (BL-PC), from PC adsorption, was used enzymes resulting biomimetic systems compared several other layers including a monolayer (ML-PC), mixed phosphatidylcholine/octyl-galactoside layer (PC-OGal), cetyltrimethylammonium (CTA), also bare surface. Interfacial characteristics these investigated with focus packing density, hydrophilicity/hydrophobicity, surface charge, which are being considered key points enzyme stabilization their biological activity. Cytoplasmic membrane-bound oxidase, served model enzymes, we...
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