EVALUATION OF A NEW METHOD FOR THE PREPARATION OF HOMOGENEOUS MYCELIAL SUSPENSIONS

摘要: Abstract Guidry, D. J. (Louisiana State University, New Orleans) and G. H. Trelles. Evaluation of a new method for the preparation homogeneous mycelial suspensions. Bacteriol. 83:53–60. 1962.—An all-glass tissue homogenizer (providing both conical cylindrical grinding surfaces) was employed in Pellets mycelium from constant shake liquid culture were submitted to various periods time. The effect time on optical density homogenate, hyphal fragment size, viability fungus determined. A 4 min found yield suspensions which 89% fragments contained four or less than cells. Optical used as basis standard samples inoculum measurement growth. Preparation light transmittance curve facilitates any desired concentration. Although plate colony counts same flask showed satisfactory agreement, there appreciable variation different flasks. The growth flasks receiving equal also Results indicate that, at concentrations an 0.301, difference 15% more two (10 per sample) can be accepted significant. Some factors influencing well have been Ways experimental errors kept minimum are discussed.