RAPID QUANTITATIVE METHOD FOR MEASURING THE SENSITIVITY OF BLASTOMYCES DERMATITIDIS TO FUNGISTATIC AGENTS

摘要: Guidry, D. J. (Louisiana State University School of Medicine, New Orleans) and Findlay Maier. Rapid quantitative method for measuring the sensitivity Blastomyces dermatitidis to fungistatic agents. Bacteriol. 85:504–508. 1963.—Isolates (yeast phase) were grown in continuous shake liquid culture. When sufficient cells added impart an initial optical density 0.100 inoculated medium, growth was rapid, time required assay agents reduced 48 hr. Standard curves on light transmittance direct cell count facilitated preparation inoculum measurement growth. Generation determined various phases B. dermatitidis, data applied selection optimal period assaying Four isolates tested their amphotericin 2-hydroxystilbamidine. An B concentration 1.4 μg/ml maximal inhibition all tested; there differences among lower concentrations drug. A 2-hydroxystilbamidine 62.5 two isolates; remaining a 125 μg/ml. latent occurred when cultures dermatitidis. This period, together with generation yeast cells, degree which could be attained.