Dermatophytes growth curve and in vitro susceptibility test: a broth micro-titration method

摘要: The introduction of systemic antifungal drugs which act upon different targets is the main issue in-vivo resistance control. Different factors, such as growth curve phase, quality specimen, quantity inoculum, temperature, pH, culture medium composition, incubation duration and solvent, are believed important factors affecting minimum inhibitory concentration (MIC) value to most agents. We assayed an in vitro susceptibility test with 40 isolates dermatophytes: Microsporum canis, Trichophyton rubrum, mentagrophytes Epidermophyton floccosum against griseofulvin, fluconazole, itraconazole terbinafine, using guidelines M38-P document approved by NCCLS. determined curves, estimate specific rate (µ max) generation time (G) each dermatophyte, dry weight spectrophotometry methods. demonstrate that, at 192 h, all fungi tested had a constant we considered this ...