Methodology for Studying the Compartments of the Human Breast

摘要: A shortcoming of using human breast tissue for molecular analysis is the heterogeneous nature sample. To resolve this, various microdissection techniques have been employed to obtain homogeneous cells, such as manual scraping with scalpel blades, needles, or other probes positively select cells interest. These are limited because poor delineation and high susceptibility contamination from dissimilar cells. Infrared laser capture (LCM), developed at National Institute סf Health, has become increasingly commercially available in last two decades twentieth century [1–3]. Laser microdissection, also called (LMD), a method isolating specific regions interest tissue, organisms. This technique enables researchers investigate DNA/RNA proteins tissue. LCM consists an LMD system, camera, software used collect areas The basic principle involves which fuses desired material onto specialized cap can be then close over 0.5 mL microcentrifuge tube. Before performed, paraffin-embedded (PET) sectioned regular microtome, cryostat create histological sections frozen placed membrane slide freshly hemotoxylin eosin (H&E) stained. After stained, sample set 100 % EtOH followed by air drying.