Comparison of Specimens for Detection of Porcine Reproductive and Respiratory Syndrome Virus Infection in Boar Studs

摘要: Porcine reproductive and respiratory syndrome virus (PRRSV)-contaminated semen from boars is a route of transmission to females, early detection PRRSV infection in key component sow farm biosecurity. The purpose this study was determine the optimum diagnostic specimen(s) for acute boars. Individually housed (n = 15) were trained oral fluid collection then vaccinated with commercial modified live vaccine. Starting on day vaccination 14 days thereafter, specimens collected daily all 15 subdivided into three groups 5, serum, blood swabs 'frothy saliva' at time 3-day rotation. Frothy saliva, derived submandibular salivary gland, produced by aroused Semen centrifuged, supernatant cell fractions tested separately. All samples randomly ordered real-time quantitative reverse-transcription polymerase chain reaction assay (rRT-PCR) antibody ELISA. In study, comparison swab, rRT-PCR results found no statistically significant differences onset or proportion positives, but serum numerically superior fluids detection. Serum provided identical ≥ 5 post-vaccination. Likewise, frothy saliva equivalent, again showing numerical advantage. These showed that highest assurance providing PRRSV-negative farms should be based testing collection. This approach can augmented sampling random selection uncollected provide valid surveillance boar stud.