Studies on dihydronicotinamide adenine dinucleotide ubiquinone reductase. I. Assay of ubiquinone reductase activity in submitochondrial particles and extracts.
作者： Richard L. Pharo , Louis A. Sordahl , S.R. Vyas , D.R. Sanadi
DOI: 10.1016/S0021-9258(18)99712-7
关键词:
摘要: Abstract Mitochondrial particles prepared by exposing mitochondria to sonic oscillation catalyzed the oxidation of reduced nicotinamide adenine dinucleotide ubiquinone-6, ubiquinone-10, lower homologues ubiquinone, and menadione. Ubiquinone-6 reduction activity was inhibited completely rotenone amytal. Menadione also inhibited, but only partially, suggesting multiple sites interaction menadione with respiratory chain. The extraction a soluble ubiquinone reductase from mitochondrial is described. assay conditions for measuring particulate preparations are described in detail. assay, which carried out heterogeneous system containing suspension, sensitive variety factors.
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