Molecular, biochemical, and morphometric characterization of Fasciola species potentially causing zoonotic disease in Egypt

摘要: Fascioliasis is an important disease caused by Fasciola hepatica and gigantica. The distributions of both species overlap in many areas Asia Africa including Egypt. Fifty adult worms were collected from livers cattle sheep slaughtered abattoirs, Cairo, They subjected to morphological metric assessment external features fresh adults, internal anatomy stained mounted worms, determination electrophorezed bands crude homogenates using SDS-PAGE, molecular characterization species-specific DNA segments RFLP-PCR. It was found that the correlation between conventional morphology its morphotype statistically significant (P value = 0.00). Using 13 detected among genotypes (35.7, 33.6, 32.4, 29.3, 27.5, 26, 24.4, 23, 21.45, 19, 16.75, 12.5, 9.1 kDa).The most prevalent with a weight 19 kDa. Bands common for species, but protein could not distinguish F. result PCR amplification selected 28S rDNA fragment designed primer set yielded 618 bp long products Different band patterns generated after digestion segment enzyme AvaII obtained showed length 529, 62, 27 bp, while gigantica 322, 269, obtained. Genotyping revealed no equivocal results. parameters spp. endemic Egypt evaluated versus genotyping. concluded assessments useful differentiation due extensive relative ranges. Similar conclusion reached concerning where banding mostly similar. In contrast, genotyping RFLP-PCR gave consistent results clear two species. Considering implications proper speciation parasites on clinical evaluation, therapy, epidemiology, control measures, currently revised basis. presently used tool therefore recommended further study help draw map geographical distribution