作者: Jie Wu , Chongguang Yang , Liping Lu , Wanqin Dai
DOI: 10.1016/J.TUBE.2018.10.012
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摘要: Abstract Objective Detection of tuberculosis laboratory cross-contamination using whole-genome sequencing. Methods A total 22 M. strains with high genotypic homology from one hospital were collected during the drug resistance surveillance. Genome sequencing and epidemiological investigation conducted to determine occurrence cross-contamination. Results The pair wise comparison between genomes in each cluster indicated that 15 (71.4%) 21 available genomic data had no SNP differences at least other strain within same cluster. analysis specimen collection time found that, among 16 on day, 14 (87.5%) them another strain; meanwhile, whose distance was 0, 93.3% (14/15) time, suggesting these findings most likely caused by cross contamination. Conclusion proportion single institute shared process period Whole genome can help