Detection of tuberculosis laboratory cross-contamination using whole-genome sequencing.

作者: Jie Wu , Chongguang Yang , Liping Lu , Wanqin Dai

DOI: 10.1016/J.TUBE.2018.10.012

关键词:

摘要: Abstract Objective Detection of tuberculosis laboratory cross-contamination using whole-genome sequencing. Methods A total 22 M. strains with high genotypic homology from one hospital were collected during the drug resistance surveillance. Genome sequencing and epidemiological investigation conducted to determine occurrence cross-contamination. Results The pair wise comparison between genomes in each cluster indicated that 15 (71.4%) 21 available genomic data had no SNP differences at least other strain within same cluster. analysis specimen collection time found that, among 16 on day, 14 (87.5%) them another strain; meanwhile, whose distance was 0, 93.3% (14/15) time, suggesting these findings most likely caused by cross contamination. Conclusion proportion single institute shared process period Whole genome can help

参考文章(11)
Zignol M, Weyer K, Rieder Hl, Wright A, Van Deun A, Drug susceptibility testing proficiency in the network of supranational tuberculosis reference laboratories. International Journal of Tuberculosis and Lung Disease. ,vol. 15, pp. 116- 124 ,(2011)
Marcelo de C. Ramos, Hanna Soini, Glaucia C. Roscanni, Monica Jaques, Maria C. Villares, James M. Musser, Extensive Cross-Contamination of Specimens with Mycobacterium tuberculosis in a Reference Laboratory Journal of Clinical Microbiology. ,vol. 37, pp. 916- 919 ,(1999) , 10.1128/JCM.37.4.916-919.1999
Mohammad Asgharzadeh, Hossein Samadi Kafil, Sajjad Ghorghanlu, Mahya Pourostadi, Laboratory cross-contamination of Mycobacterium tuberculosis in Northwest of Iran Egyptian Journal of Chest Diseases and Tuberculosis. ,vol. 64, pp. 665- 669 ,(2015) , 10.1016/J.EJCDT.2015.03.018
Qian Gao, Ying Peng, Chongguang Yang, Xia Li, Tao Luo, Fabin Li, Multiple samples improve the sensitivity for detection of mixed Mycobacterium infections Tuberculosis. ,vol. 93, pp. 548- 550 ,(2013) , 10.1016/J.TUBE.2013.06.002
F. A. Drobniewski, S. Hoffner, S. Rusch-Gerdes, G. Skenders, V. Thomsen, , Recommended standards for modern tuberculosis laboratory services in Europe European Respiratory Journal. ,vol. 28, pp. 903- 909 ,(2006) , 10.1183/09031936.06.00084906
Chongguang Yang, Xin Shen, Ying Peng, Rushu Lan, Yuling Zhao, Bo Long, Tao Luo, Guomei Sun, Xia Li, Ke Qiao, Xiaohong Gui, Jie Wu, Jiying Xu, Fabin Li, Dingyue Li, Feiying Liu, Mei Shen, Jianjun Hong, Jian Mei, Kathryn DeRiemer, Qian Gao, Transmission of Mycobacterium tuberculosis in China: A Population-Based Molecular Epidemiologic Study Clinical Infectious Diseases. ,vol. 61, pp. 219- 227 ,(2015) , 10.1093/CID/CIV255
N E Dunlap, R H Harris, W H Benjamin, J W Harden, D Hafner, Laboratory Contamination of Mycobacterium tuberculosis Cultures American Journal of Respiratory and Critical Care Medicine. ,vol. 152, pp. 1702- 1704 ,(1995) , 10.1164/AJRCCM.152.5.7582316
Christopher JL Murray, Joshua A Salomon, None, Modeling the impact of global tuberculosis control strategies Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 13881- 13886 ,(1998) , 10.1073/PNAS.95.23.13881
L. Fitzpatrick, C. Braden, W. Cronin, J. English, E. Campbell, S. Valway, I. Onorato, Investigation of Laboratory Cross-Contamination of Mycobacterium tuberculosis Cultures Clinical Infectious Diseases. ,vol. 38, ,(2004) , 10.1086/382076
FA Drobniewski, M Caws, A Gibson, D Young, Modern laboratory diagnosis of tuberculosis. Lancet Infectious Diseases. ,vol. 3, pp. 141- 147 ,(2003) , 10.1016/S1473-3099(03)00544-9