Physiological correlates of chill susceptibility in Lepidoptera.

摘要: The majority of insects enter a state reversible coma if temperature is lowered sufficiently. If the cold treatment not too severe these recover gradually when returned to benign temperatures in time-dependent manner that often depends on duration and intensity exposure. Previous studies have associated phenotypes changes membrane potential (Vm) ion balance, especially hemolymph [K+] known be importance for recovery time. In present study we examined this link three species Lepidoptera as from order are possess resting would severely compromise Vm other insects. Specifically, exposed larval adult Manduca sexta, Bombyx mori, Heliconius cydno stressful (0°C) extended periods Subsequently measured chill time (CCRT), ion- water muscle Vm. As expected find high [Na+] low compared most insect species. Muscle depolarised considerably during acute exposure, but did so was with balance. However, prolonged exposure coincided an increase further depolarisation which correlated well prolongation CCRT. Combined demonstrates how different ionic compositions generally suffer similar consequences stress species, such tolerance chill-susceptible within also intimately linked maintenance balance polarisation.