Clonal analysis of vertebrate myogenesis

作者: Jennifer Seed , Stephen D. Hauschka

DOI: 10.1016/0012-1606(88)90264-3

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摘要: The effect of bovine fibroblast growth factor (FGF) on the in vitro differentiation various stage-specific populations skeletal muscle colony-forming (MCF) cells from developing chick wing bud was examined. results show that FGF (3 ng/ml daily) delays onset MCF obtained Day 4-12 buds by about 1 day; but, addition, demonstrate a subset derived stage 23-27 (Day 4-5) embryos require for myogenic differentiation. FGF-dependent attach and grow absence FGF, but do not differentiate unless given within 1-3 days after inoculation. Thus, between stages 23 27 population contains discrete subclasses are dependent others independent. Both found two previously classified cell populations, early late cells. independent present until 25, which only FGF-independent subclass persists. Similarly, both -independent 25 27, remains 31. mechanisms responsible relative changes proportions requirements understood. In while is required, there no evidence suggesting triggers terminal subclasses.

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