A Gly65Val substitution in an actin, GhACT_LI1, disrupts cell polarity and F-actin organization resulting in dwarf, lintless cotton plants.
作者： Gregory N. Thyssen , David D. Fang , Rickie B. Turley , Christopher B. Florane , Ping Li
DOI: 10.1111/TPJ.13477
关键词:
摘要: Actin polymerizes to form part of the cytoskeleton and organize polar growth in all eukaryotic cells. Species with numerous actin genes are especially useful for dissection molecular function due redundancy neofunctionalization. Here, we investigated role a cotton (Gossypium hirsutum) gene organization filaments lobed cotyledon pavement cells highly elongated single-celled trichomes that comprise lint fibers. Using mapping-by-sequencing, virus-induced silencing, modeling, identified causative mutation dominant dwarf Ligon lintless Li1 short fiber mutant as single Gly65Val amino acid substitution polymerization domain an gene, GhACT_LI1 (Gh_D04G0865). We observed altered cell morphology disrupted F-actin plant by confocal microscopy. Mutant leaf lacked interdigitation lobes did not uniformly decorate nuclear envelope. While wild-type trichome contained long longitudinal cables, accumulated disoriented transverse cables. The polymerization-defective allele plants likely disrupts processive elongation F-actin, resulting disorganized reduced polarity, which accounts action diverse pleiotropic effects associated mutation. Lastly, propose model account these effects, underscore roles determining shape growth.
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