Laboratory-scale investigations into the use of extremely thermophilic proteinases for cleaning ultrafiltration membranes fouled during whey processing

摘要: Abstract The potential of proteolytic enzymes from extremely thermophilic eubacteria to clear ultrafiltration membranes fouled during the processing whey was investigated. were initially tested for their ability hydrolyse freeze-dried preparations material removed membranes. Under conditions used, serine proteinases Thermus strains more efficient than metalloproteinase Bacillus strain EA.1, and subsequently investigated membrane discs using purpose-built laboratory scale equipment. proteinase Rt4.1A proved most effective gave a linear increase water flux with time when used treat at 70°C. rate clearing final levels obtained enzyme alone lower those alkaline-EDTA. performance was, however, synergistically enhanced anionic detergent sodium dodecyl sulphate in combination enzyme.