Adaptation of Fusarium graminearum to tebuconazole yielded descendants diverging for levels of fitness, fungicide resistance, virulence, and mycotoxin production.

摘要: Azole fungicides play a prominent role for reliable plant disease management. However, quantitative azole resistance has been shown to develop in fungal pathogens, including Fusarium graminearum, the causal agent of head blight (FHB). Due widespread application fungicides, may accumulate higher degrees field populations over time. Although are components FHB control, little effort made investigate F. graminearum. We allowed graminearum strain NRRL 13383 adapt an fungicide vitro, applying strongly growth-reducing but sublethal dose tebuconazole. Two morphologically distinguishable azole-resistant phenotypes were recovered that differed with regard levels fitness, resistance, virulence, and mycotoxin production. Isolates adapted "phenotype 1" exhibited azole-specific cross-resistance, whereas 2" isolates displayed phenomenon multidrug because sensitivity amine was also affected. Assessment individual infected spikelets contents by high-performance liquid chromatography mass spectrometry DNA polymerase chain reaction indicated some produced significantly nivalenol per biomass than strain.