Novel cellulases from an extremophilic filamentous fungi Penicillium citrinum : production and characterization
作者： Tanmay Dutta , Rupam Sahoo , Rajib Sengupta , Sougata Sinha Ray , Arindam Bhattacharjee
DOI: 10.1007/S10295-008-0304-2
关键词:
摘要: The enzymatic hydrolysis of cellulose has potential economical and environment-friendly applications. Therefore, discovery new extremophilic cellulases is essential to meet the requirements industry. Penicillium citrinum (MTCC 6489) that was previously isolated from soil in our laboratory, produced alkali tolerant thermostable cellulases. Endoglucanase filter paper activity hydrolase (FPAse) production P. were studied using wheat bran substrate solid state submerged culture. Zymogram analysis endoglucanase revealed presence two isoforms differing molecular weight. One them 90 kDa other one 38 kDa. Partially purified showed different peaks at pH 5.5 8.0, respectively, its optima curve. But FPase only peak (at 6.5) Cellulase nature. present work reports for first time, stable cellulase fungus citrinum. Thermostable may have effectiveness as additives laundry detergents.
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