miR‐224 promotion of cell migration and invasion by targeting Homeobox D 10 gene in human hepatocellular carcinoma

摘要: Background and Aim MicroRNAs (miRNAs) are small noncoding RNA molecules that control target gene expression implicated in the regulation of diverse cellular pathways. In our previous research, we have demonstrated miR-224 was overexpressed liver cancer cells tissues, which an important factor cell migration invasion. This study aimed to further explore regulatory mechanism invasion cells. Methods A luciferase reporter assay used confirm HOXD10 a direct miR-224. Quantitative reverse transcriptase-polymerase chain reaction, Western blotting, Transwell migration, Matrigel assays were performed clarify molecular human hepatocellular carcinoma (HCC). Results  (i) The strongly upregulated MHHC97H MHCC97L cells, its level significantly associated with invasive potential. (ii) confirmed be Compared normal tissues had lower HCC inversely regulated (iii) promoted tumor invasion-associated proteins p-PAK4 MMP-9 by directly targeting HOXD10. Conclusion Our findings suggest previously undescribed pathway miR-224/HOXD10/p-PAK4/MMP-9 signaling contributes provides new biotarget for treatment.