MicroRNA-224 Induces G1/S Checkpoint Release in Liver Cancer.
作者： Fangmei An , Alexandru Olaru , Esteban Mezey , Qing Xie , Ling Li
DOI: 10.3390/JCM4091713
关键词:
摘要: Profound changes in microRNA (miR) expression levels are frequently found liver cancers compared to the normal liver. In this study, we evaluate of miR-224 human HCC and CCA, as well its downstream targets affected pathways. We show that is upregulated a large cohort similar upregulation HCC. For purpose studying roles enforced cells. mRNA arrays followed by Ingenuity Pathway Analysis (IPA)-identified putative molecules pathways miR-224. Phenotypically, report increases growth rate cholangiocytes, CCA cell lines, lines. addition, identified, an unbiased fashion, one major biologic processes Gap1 (G1) Synthesis (S) transition checkpoint release. next identified p21, p15, CCNE1 confirmed coordinated downregulation results increased phosphorylation Retinoblastoma (Rb) with resulting G1/S Our data suggest master regulator cycle progression, overexpression release accelerated growth.
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