Bisulfite Sequencing in Preimplantation Embryos: DNA Methylation Profile of the Upstream Region of the Mouse ImprintedH19Gene

摘要: In this study we describe a modification of the bisulfite genomic sequencing protocol that enables detection methylation from as few five diploid cells preimplantation mouse embryos. We have used to profile putative imprinting element upstream H19 gene at several stages embryonic development, including fertilized oocytes and two-cell The has recently been described extensively for midgestation embryos, but remains poorly characterized despite widespread changes in DNA occurring time. studied 35 CpG sites spanning two regions within found an overall pattern allele-specific was maintained all developmental examined, However, not absolute fashion subsequent first cell division, with clear flux between partial de novo maternal allele demethylation paternal allele. Our findings highlight dynamics early embryo suggest it is level responsible maintenance individual sites.