Degradation products of extracellular matrix affect cell migration and proliferation.

摘要: Biologic scaffolds composed of extracellular matrix (ECM) are utilized in numerous regenerative medicine applications to facilitate the constructive remodeling tissues and organs. The mechanisms by which host response occurs not fully understood, but recent studies suggest that both constituent growth factors biologically active degradation products derived from ECM play important roles. objective present study was determine if scaffold materials vitro methods biochemically physiologically relevant can yield possess chemotactic and/or mitogenic activities for differentiated mammalian endothelial cells undifferentiated multipotential progenitor cells. harvested porcine urinary bladder degraded enzymatically with pepsin/hydrochloric acid or papain. were tested chemoattractant properties utilizing either 48-well chemotaxis filter migration microchambers fluorescence-based assays, cell proliferation assays. Results showed possessed same inhibited These findings support concept bioscaffolds modulators recruitment appropriate types during process remodeling.