Synthetic Erythropoietic Proteins: Tuning Biological Performance by Site-Specific Polymer Attachment
作者： Shiah-Yun Chen , Sonya Cressman , Feng Mao , Haiyan Shao , Donald W. Low
DOI: 10.1016/J.CHEMBIOL.2005.01.017
关键词:
摘要: Chemical synthesis in combination with precision polymer modification allows the systematic exploration of effect protein properties, such as charge and hydrodynamic radius, on potency using defined, homogeneous conjugates. A series polymer-modified synthetic erythropoiesis proteins were constructed that had a polypeptide chain similar to amino acid sequence human erythropoietin but differed significantly number type attached polymers. The analogs from +5 -26 at neutral pH varied molecular weight 30 54 kDa. All active an vitro cell proliferation assay. However, vivo was found be strongly dependent overall size. trends observed this study may serve starting points for construction more potent EPO future.
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