Citraconic anhydride: a new antigen retrieval solution

摘要: Background: The introduction of heat-induced antigen retrieval has been a major milestone in diagnostic immunohistochemistry, enabling the application many antibodies to fixed paraffin-embedded tissue sections. A number important variables affect preservation antigens, among which are analytical including methodology. Temperature retrieval, duration heating, source heat, pH and nature solution more pivotal results. Citrate buffer at 6.0 become widely embraced as universal fixative but some remain capricious yield poor staining results.Methods: This study examines recent suggestion that citraconic anhydride may be suitable reagent. Immunostaining 65 commonly employed following microwave 0.05% for 10 minutes 98°C was compared with consecutive tissues sections subjected ...