Resistance of virus to extinction on bottleneck passages: Study of a decaying and fluctuating pattern of fitness loss

摘要: RNA viruses display high mutation rates and their populations replicate as dynamic complex mutant distributions, termed viral quasispecies. Repeated genetic bottlenecks, which experimentally are carried out through serial plaque-to-plaque transfers of the virus, lead to fitness decrease (measured here diminished capacity produce infectious progeny). Here we report an analysis evolution several low foot-and-mouth disease virus clones subjected 50 transfers. Unexpectedly, decrease, rather than being continuous monotonic, displayed a fluctuating pattern, was influenced by both state host cell shown effects recent passage history. The amplitude fluctuations increased decreased, resulting in remarkable resistance extinction. Whereas frequency distribution control (independent) experiments follows log-normal distribution, probability values evolving bottlenecked fitted Weibull distribution. We suggest that multiple functions genomic its encoded proteins, mutational pressure, interact with cellular components this nontrivial, pattern.