Pathogenesis of porcine epidemic diarrhea virus isolate (US/Iowa/18984/2013) in 3-week-old weaned pigs

摘要: Porcine epidemic diarrhea virus (PEDV) is associated with clinical in naive swine of all ages. This report describes timing antibody generation and disease progression following infection a US PEDV isolate by assessing fecal viral shedding, morphometric analysis intestinal lesions, magnitude immunohistochemical staining. Sixty-three, 3-week-old pigs were randomly allocated into control (n=27) challenged (n=36) groups. Challenged administered 1 mL × 10(3) PFU/mL US/Iowa/18984/2013 oro-gastric gavage. Three four necropsied on days post-inoculation (dpi) 1, 2, 3, 4, 7, weekly thereafter, until study termination dpi 35. Clinical disease, body weight, temperature monitored during the period. Diarrhea was observed beginning for some affecting majority 6 subsiding 10. Average daily gain significantly lower (P<0.001) one week post-infection pigs. detected feces PCR continued subset 24. PEDV-specific antigen villous enterocytes immunohistochemistry (IHC) 14. Microscopic lesions included severe diffuse atrophic enteritis reduced length 7. Under conditions this study, shedding IHC staining can precede continue beyond observation signs, thus increasing risk transmission.