Feasibility, limitation and possible solutions of RNAi-based technology for insect pest control.

摘要: Numerous studies indicate that target gene silencing by RNA interference (RNAi) could lead to insect death. This phenomenon has been considered as a potential strategy for pest control, and it is termed RNAi-mediated crop protection. However, there are many limitations using RNAi-based technology with the effectiveness selection reliable double-strand (dsRNA) delivery being two of major challenges. With respect selection, at present, use homologous genes genome-scale high-throughput screening main strategies adopted researchers. Once identified, dsRNA can be delivered micro-injection or feeding dietary component. micro-injection, which most common method, only used in laboratory experiments. Expression dsRNAs directed against transgenic plants spraying reagents have shown induce RNAi effects on insects. Hence, protection new-generation complementary method existing control strategies; however, further development improve efficacy range species affected necessary. In this review, we summarized current research management. Current progress proven tool designing new generation measures. To accelerate its practical application protection, study uptake mechanisms based knowledge physiology biochemistry needed.