Activity Test of Dexamethasone Therapy to Humoral Immunity in Balb/c Mice with Biliary Atresia

摘要: The prevalence of biliary atresia is 1:5.000-8.000 live births. Kasai portoenterostomy a reliable treatment, but liver damage continues. Dexamethasone (corticosteroid) evolved into commonly used therapy and believed to improve clinical outcomes in atresia. objective this study was determine the effect dexamethasone on absolute number IgA, IgD, IgE, IgG, IgM mice Balb/c model Murine obtained by injection 20 μL PBS containing 10 6 pfu Rhesus rotavirus (RRV) at <24 hours age. Injection with dose 0.5 mg/kg body weight done day 7 14, 14 21 after virus injection. Spleen taken for analysis flow cytometry. Data were tested Kruskal-Wallis test, then follow up Man-Whitney test assess   the difference SPSS 16.0 Windows . Here, we show significant immunoglobulin molecule both termination (p<0.05). Subcutaneously RRV (Rhesus Rotavirus) aged capable stimulate production immunoglobulin, especially third week. This can be evidenced an increase IgG. glucocorticoid that plays role as immunosuppressant ability decreasing IgM, IgG levels termination. Furthermore showed stimulating IgA Keywords : baby mice, Biliary atresia, Corticosteroid, Dexamethasone, Rotavirus