Immunization of mice with recombinant gp41 in a systemic prime/mucosal boost protocol induces HIV-1-specific serum IgG and secretory IgA antibodies.

摘要: Abstract We tested the immunogenicity in mice of a recombinant fusion protein (gp41HA) consisting ectodomain HIV-1 IIIB envelope glycoprotein gp41 fused to fragment influenza virus HA2 hemagglutinin protein. An intraperitoneal prime followed by intranasal or intragastric boosts with gp41HA induced high concentrations serum IgG antibodies and fecal IgA that reacted viral lysate were cross-reactive MN lysate. By indirect immunofluorescence, from immunized also shown recognize acetone-fixed human peripheral blood mononuclear cells infected either syncytium-inducing (SI) non-syncytium-inducing (NSI) North American field isolates, but not uninfected cells. Thus, this antigen may be useful prime/boost immunization protocols designed induce systemic mucosal multiple primary isolates.