The requirement of zinc and calcium ions for functional MMP activity in demineralized dentin matrices
作者： Arzu Tezvergil-Mutluay , Kelli A Agee , Tomohiro Hoshika , Marcela Carrilho , Lorenzo Breschi
DOI: 10.1016/J.DENTAL.2010.07.006
关键词:
摘要: The progressive degradation of resin–dentin bonds is due, in part, to the slow collagen fibrils hybrid layer by endogenous matrix metalloproteinases (MMPs) dentin matrix. In vitro durability studies, storage medium composition might be important because optimum activity MMPs requires both zinc and calcium. Objective This study evaluated effect different media on changes stiffness, loss dry weight or solubilization from demineralized beams incubated for up 60 days. Methods Dentin (1 mm × 2 mm × 6 mm) were completely 10% phosphoric acid. After baseline measurements mass elastic modulus (E) (3-point bending, 15% strain) divided into 5 groups (n = 11/group) at 37 °C either containing calcium designated as complete (CM), calcium-free medium, zinc-free a doubled-zinc water. Beams retested 3, 7, 14, 30, days incubation. incubation was hydrolyzed with HCl quantitation hydroxyproline (HOP) an index MMPs. Data analyzed using repeated measures ANOVA. Results Both time showed significant effects E, HOP release (p < 0.05). CM resulted relatively rapid (p < 0.05) decreases increasing amounts loss. content experimental also increased but significantly lower than control recommended medium. Conclusions The solutions used age should buffered that contain zinc. common use water aging may underestimate hydrolytic
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