ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity
作者： Tomohiko Ohta , Jennifer J. Michel , Arndt J. Schottelius , Yue Xiong
DOI: 10.1016/S1097-2765(00)80482-7
关键词:
摘要: We have identified two highly conserved RING finger proteins, ROC1 and ROC2, that are homologous to APC11, a subunit of the anaphase-promoting complex. ROC2 commonly interact with all cullins while APC11 specifically interacts APC2, cullin-related APC subunit. YeastROC1 encodes an essential gene whose reduced expression resulted in multiple, elongated buds accumulation Sic1p Cln2p. immunocomplexes can catalyze isopeptide ligations form polyubiquitin chains E1- E2-dependent manner. mutations completely abolished their ligase activity without noticeable changes associated proteins. Ubiquitination phosphorylated I kappa B alpha be catalyzed by immunocomplex vitro. Hence, combinations ROC/APC11 cullin proteins potentially constitute wide variety ubiquitin ligases.
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