Genome-Wide Analysis of Histidine Repeats Reveals Their Role in the Localization of Human Proteins to the Nuclear Speckles Compartment

摘要: Single amino acid repeats are prevalent in eukaryote organisms, although the role of many such sequences is still poorly understood. We have performed a comprehensive analysis proteins containing homopolymeric histidine tracts human genome and identified 86 that contain stretches five or more histidines. Most them endowed with DNA- RNA-related functions, and, addition, there an overrepresentation expressed brain and/or nervous system development. An their subcellular localization shows 15 22 nuclear accumulate subcompartment known as speckles. This lost when repeat deleted, significantly, closely related paralogous without also fail to localize Hence, tract appears be directly involved targeting this compartment. The removal DNA-binding domains treatment RNA polymerase II inhibitors induces re-localization several polyhistidine-containing from nucleoplasm These findings highlight dynamic relationship between sites transcription Therefore, we define novel signal for speckles, suggest these way generating evolutionary diversification gene duplicates. data contribute our better understanding physiological single proteins.