[23] Isolation of cyanobacterial plasma membranes

摘要: Publisher Summary This chapter focuses on the isolation of cyanobacterial plasma membranes. Cyanobacteria are classified as gram-negative bacteria, in which cell envelope is composed outer and inner (plasma or cytoplasmic) membranes with a peptidoglycan layer between. In cytoplasm thylakoid membranes, site light capturing, electron transport, ATP synthesis. The represent most total cellular this situation made it difficult for long time to purify from cyanobacteria. Subsequently developed methods isolate essentially same their method, although some modifications employed such using French pressure disruption cells flotation centrifugation Plasma thus prepared can be identified by microscopy also labeling intact prior membrane-impermeant protein marker diazobenzene [ 35 S]sulfonate.