MicroRNA-122 inhibits proliferation and invasion in gastric cancer by targeting CREB1.

摘要: MicroRNA-122 (miR-122) has been implicated in tumor development and progression various types of cancers. However, the biological function regulatory mechanisms miR-122 gastric cancer (GC) remain largely unknown. We aimed to determine role underlying mechanism GC. Real time quantitative RT-PCR (qRT-PCR) was performed detect expression GC tissues cell lines. CCK8, wound healing, transwell assays were conducted effect on proliferation, migration, invasion, respectively. Target molecules identified by luciferase activity, RT-PCR, western blotting. found that significantly decreased both lines reduced associated with aggressive clinicopathological features patients. also overexpression markedly inhibited invasion In addition, cAMP responsive element binding protein 1 (CREB1) as a direct target miR-122, its negatively correlated (r = -0.711, P < 0.001). CREB1overexpression rescued suppressive invasion. Moreover, we demonstrated tumorigenesis vivo repressing CREB1 expression. These findings suggest might suppressor could serve promising candidate for therapeutic applications regarding treatment.