[71] The isolation of endothelial cells from normal human and bovine brain

摘要: Publisher Summary The isolation of endothelial cells from brain is carried out by disrupting tissue followed the selective long filamentous (unicellular capillaries) filtration and foam fractionation. In presence calcium ATP, remain intact metabolically active over several days if maintained in cold (+ 40°C). These do not fragment completely during homogenization, they retail their multicellular structures. cell masses are connected end-to-end with a common lumen. chains can be filtered off on glass or Sephadex columns contaminating structures such as nuclei, mitochondria, etc. Final purification achieved fractionation media high surface tension.